Anti-AFP单克隆说明书

mouseanti-afp单克隆说明书
from:abcam
catalognumber:rs-4708r
quantitysize:100ul(dilutewithph7.40.01mpbsorantibodydiluent)
background:
alpha1fetoproteinisamajorplasmaproteinproducedbytheyolksacandtheliverduringfetallife.alphafetoproteinexpressioninadultsisoftenassociatedwithhepatomaorteratoma.however,hereditarypersistanceofalpha-fetoproteinmayalsobefoundinindividualswithnoobviouspathology.theproteinisthoughttobethefetalcounterpartofserumalbumin,andthealphafetoproteinandalbumingenesarepresentintandeminthesametranscriptionalorientationonchromosome4.alphafetoproteinisfoundinmonomericaswellasdimericandtrimericforms,andbindscopper,nickel,fattyacidsandbilirubin.thelevelofalphafetoproteininamnioticfluidisusedtomeasurerenallossofproteintoscreenforspinabifidaandanencephaly.expressionhasbeendocumentedinhumanadrenal,liver,ovary,testis,andpancreas.estshavebeenisolatedfromnormalhumanbrain,liver/spleen,embryoanduterustissuelibraries.
function:
bindscopper,nickel,andfattyacidsaswellas,andbilirubinlesswellthan,serumalbumin.onlyasmallpercentage(lessthan2%)ofthehumanafpshowsestrogen-bindingproperties.
specificity:anti-afpisamousemonoclonalantibody
specificforanti-afpofrat,human,mouse,
-useforwesternblotting,elisa,immunoprecipitationandimmunohistochemistry
proteingaffinitychromatographypurification,purity:>95%isotype:iggmolwt:65kda
application:
wb=1:100-500elisa=1:500-1000ihc-p=1:100-500ihc-f=1:100-500optimaldilutions/concentrationsshouldbedeterminedbytheenduser..klhconjugatedsyntheticpeptidederivedfromhumanafp
storage:storeat-20℃foroneyear.avoidrepeatedfreeze/thawcycles.thelyophilizedantibodyisstableatroomtemperatureforatleastonemonthandforgreaterthanayearwhenkeptat-20℃.whenreconstitutedinsterileph7.40.01mpbsordiluentofantibody,theantibodyisstableforatleastsixweeksat2-4℃
importantnote:thisproductassuppliedisintendedforresearchuseonly,notforuseinhuman,therapeuticordiagnosticapplications.

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Anti-AFP单克隆说明书
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