红细胞生成素受体（EPOR）检测试剂盒说明书
红细胞生成素受体(epor)检测试剂盒
检测范围 0.156-10ng/ml 灵敏度 0.066ng/ml
样本类型 serum, plasma, tissue homogenates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
红细胞生成素受体(epor)检测试剂盒 elisa kit for erythropoietin receptor (epor)
for in vitro and research use only, not for use in clinical diagnostic procedures!
organism species homo sapiens (human)
product no. sea027hu
sample type serum, plasma, tissue homogenates and other biological fluids.
format 96-well strip plate
assay length 4.5 hours
detection range 0.156-10ng/ml the standard curve concentrations used for the elisa’s were 10ng/ml, 5ng/ml, 2.5ng/ml, 1.25ng/ml, 0.625ng/ml, 0.313ng/ml, 0.156ng/ml
sensitivity the minimum detectable dose of this kit is typically less than 0.066ng/ml.
specificity
this assay has high sensitivity and excellent specificity for detection of erythropoietin receptor (epor).
no significant cross-reactivity or interference between erythropoietin receptor (epor) and analogues was observed. recovery
matrices listed below were spiked with certain level of recombinant erythropoietin receptor (epor) and the recovery rates were calculated by comparing the measured value to the expected amount of erythropoietin receptor (epor) in samples.
matrix recovery range (%) average(%)
serum(n=5) 95-103 101
edta plasma(n=5) 78-93 86
heparin plasma(n=5) 90-97 94
precision
intra-assay precision (precision within an assay): 3 samples with low, middle and high level erythropoietin receptor (epor) were tested 20 times on one plate, respectively.
inter-assay precision (precision between assays): 3 samples with low, middle and high level erythropoietin receptor (epor) were tested on 3 different plates, 8 replicates in each plate.
cv(%) = sd/meanx100
intra-assay: cv<10%
inter-assay: cv<12%
linearity
the linearity of the kit was assayed by testing samples spiked with appropriate concentration of erythropoietin receptor (epor) and their serial dilutions. the results were demonstrated by the percentage of calculated concentration to the expected.
sample 1:2 1:4 1:8 1:16
serum(n=5) 81-97% 93-101% 87-104% 84-97%
edta plasma(n=5) 92-99% 96-105% 98-105% 96-105%
heparin plasma(n=5) 97-105% 78-95% 82-101% 79-90%
stability
the stability of kit is determined by the loss rate of activity. the loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
to minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. it is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. reagents and materials provided
reagents quantity reagents quantity
pre-coated, ready to use 96-well strip plate 1 plate sealer for 96 wells 4
standard 2 standard diluent 1×20ml
detection reagent a 1×120μl assay diluent a 1×12ml
detection reagent b 1×120μl assay diluent b 1×12ml
tmb substrate 1×9ml stop solution 1×6ml
wash buffer (30 × concentrate) 1×20ml instruction manual 1
assay procedure summary
1. prepare all reagents, samples and standards;
2. add 100μl standard or sample to each well. incubate 2 hours at 37oc;
3. aspirate and add 100μl prepared detection reagent a. incubate 1 hour at 37oc;
4. aspirate and wash 3 times;
5. add 100μl prepared detection reagent b. incubate 30 minutes at 37oc;
6. aspirate and wash 5 times;
7. add 90μl substrate solution. incubate 15-25 minutes at 37oc;
8. add 50μl stop solution. read at 450nm immediay.
test principle
the test principle applied in this kit is sandwich enzyme immunoassay. the microtiter plate provided in this kit has been pre-coated with an antibody specific to erythropoietin receptor (epor). standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to erythropoietin receptor (epor). next, avidin conjugated to horseradish peroxidase (hrp) is added to each microplate well and incubated. after tmb substrate solution is added, only those wells that contain erythropoietin receptor (epor), biotin-conjugated antibody and enzyme-conjugated avidin will exhibit a change in color. the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. the concentration of erythropoietin receptor (epor) in the samples is then determined by comparing the o.d. of the samples to the standard curve.
地源热泵能耗监控测温系统/地源热泵能耗地温远程监测监控系统
发酵箱的作用有哪些？
科尔奇充气接头使用
QJB型潜水搅拌机结构特点
大米品质评价标准设备RN-700
红细胞生成素受体（EPOR）检测试剂盒说明书
红外分光测油仪介绍
一机多能，解锁实验可能性：多功能熔样机的五大特点
使用光合作用仪前注意事项
英国NORGREN HB84G-4GT-AD3-RMN型减压阀故障排除方法
为什么要测量绝缘油的析气性及测量原理
数控裁板锯的操作注意事项
防爆玻璃钢离心风机能不能在高温环境下使用
紧固件摩擦系数对装配-扭矩法的影响
选择合适的VOCs治理技术，缓解环境污染难题
试试回收二手脱氨升膜蒸发器
实验室工作台安全性和方便性的设计模式
低压风机，中压风机，高压风机工作压力的三个类别
净气型试剂柜在实验室的应用
包装机如何保养和维护呢？
检测范围 0.156-10ng/ml 灵敏度 0.066ng/ml
样本类型 serum, plasma, tissue homogenates and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
红细胞生成素受体(epor)检测试剂盒 elisa kit for erythropoietin receptor (epor)
for in vitro and research use only, not for use in clinical diagnostic procedures!
organism species homo sapiens (human)
product no. sea027hu
sample type serum, plasma, tissue homogenates and other biological fluids.
format 96-well strip plate
assay length 4.5 hours
detection range 0.156-10ng/ml the standard curve concentrations used for the elisa’s were 10ng/ml, 5ng/ml, 2.5ng/ml, 1.25ng/ml, 0.625ng/ml, 0.313ng/ml, 0.156ng/ml
sensitivity the minimum detectable dose of this kit is typically less than 0.066ng/ml.
specificity
this assay has high sensitivity and excellent specificity for detection of erythropoietin receptor (epor).
no significant cross-reactivity or interference between erythropoietin receptor (epor) and analogues was observed. recovery
matrices listed below were spiked with certain level of recombinant erythropoietin receptor (epor) and the recovery rates were calculated by comparing the measured value to the expected amount of erythropoietin receptor (epor) in samples.
matrix recovery range (%) average(%)
serum(n=5) 95-103 101
edta plasma(n=5) 78-93 86
heparin plasma(n=5) 90-97 94
precision
intra-assay precision (precision within an assay): 3 samples with low, middle and high level erythropoietin receptor (epor) were tested 20 times on one plate, respectively.
inter-assay precision (precision between assays): 3 samples with low, middle and high level erythropoietin receptor (epor) were tested on 3 different plates, 8 replicates in each plate.
cv(%) = sd/meanx100
intra-assay: cv<10%
inter-assay: cv<12%
linearity
the linearity of the kit was assayed by testing samples spiked with appropriate concentration of erythropoietin receptor (epor) and their serial dilutions. the results were demonstrated by the percentage of calculated concentration to the expected.
sample 1:2 1:4 1:8 1:16
serum(n=5) 81-97% 93-101% 87-104% 84-97%
edta plasma(n=5) 92-99% 96-105% 98-105% 96-105%
heparin plasma(n=5) 97-105% 78-95% 82-101% 79-90%
stability
the stability of kit is determined by the loss rate of activity. the loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
to minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. it is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. reagents and materials provided
reagents quantity reagents quantity
pre-coated, ready to use 96-well strip plate 1 plate sealer for 96 wells 4
standard 2 standard diluent 1×20ml
detection reagent a 1×120μl assay diluent a 1×12ml
detection reagent b 1×120μl assay diluent b 1×12ml
tmb substrate 1×9ml stop solution 1×6ml
wash buffer (30 × concentrate) 1×20ml instruction manual 1
assay procedure summary
1. prepare all reagents, samples and standards;
2. add 100μl standard or sample to each well. incubate 2 hours at 37oc;
3. aspirate and add 100μl prepared detection reagent a. incubate 1 hour at 37oc;
4. aspirate and wash 3 times;
5. add 100μl prepared detection reagent b. incubate 30 minutes at 37oc;
6. aspirate and wash 5 times;
7. add 90μl substrate solution. incubate 15-25 minutes at 37oc;
8. add 50μl stop solution. read at 450nm immediay.
test principle
the test principle applied in this kit is sandwich enzyme immunoassay. the microtiter plate provided in this kit has been pre-coated with an antibody specific to erythropoietin receptor (epor). standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to erythropoietin receptor (epor). next, avidin conjugated to horseradish peroxidase (hrp) is added to each microplate well and incubated. after tmb substrate solution is added, only those wells that contain erythropoietin receptor (epor), biotin-conjugated antibody and enzyme-conjugated avidin will exhibit a change in color. the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. the concentration of erythropoietin receptor (epor) in the samples is then determined by comparing the o.d. of the samples to the standard curve.
地源热泵能耗监控测温系统/地源热泵能耗地温远程监测监控系统
发酵箱的作用有哪些？
科尔奇充气接头使用
QJB型潜水搅拌机结构特点
大米品质评价标准设备RN-700
红细胞生成素受体（EPOR）检测试剂盒说明书
红外分光测油仪介绍
一机多能，解锁实验可能性：多功能熔样机的五大特点
使用光合作用仪前注意事项
英国NORGREN HB84G-4GT-AD3-RMN型减压阀故障排除方法
为什么要测量绝缘油的析气性及测量原理
数控裁板锯的操作注意事项
防爆玻璃钢离心风机能不能在高温环境下使用
紧固件摩擦系数对装配-扭矩法的影响
选择合适的VOCs治理技术，缓解环境污染难题
试试回收二手脱氨升膜蒸发器
实验室工作台安全性和方便性的设计模式
低压风机，中压风机，高压风机工作压力的三个类别
净气型试剂柜在实验室的应用
包装机如何保养和维护呢？