NMT历*的今天丨TiO2提升黄孢原毛平革菌镉耐受力的生理证据

nmt是基因功能的活体检测技术,已被103位诺贝尔奖得主所在单位,及北大、清华、中科院使用。
nmt历*的今天
2014年12月24日,湖南大学曾光明、陈桂秋、谭琼用nmt在chemosphere上发表了标题为physiological fluxes and antioxidative enzymes activities of immobilized phanerochaete chrysosporium loaded with tio2 nanoparticles after exposure to toxic pollutants in solution的研究成果。
期刊:chemosphere
主题:tio2提升黄孢原毛平革菌镉耐受力的生理证据
标题:physiological fluxes and antioxidative enzymes activities of immobilized phanerochaete chrysosporium loaded with tio2 nanoparticles after exposure to toxic pollutants in solution
影响因子:4.208
检测指标:h+、o2、cd2+流速
作者:湖南大学曾光明、陈桂秋、谭琼
英文摘要
immobilized phanerochaete chrysosporium loaded with tio2 nanoparticles (ptns) are novel high-value bioremediation materials for adsorbing cadmium and for degrading 2,4-dichlorophenol (2,4-dcp). the real-time changes in h+ and o2 fluxes were measured using the noninvasive microtest technique (nmt).
the h+ influx increased after the addition of 2,4-dcp, and shifted to efflux following the addition of cd2+. the o2 flux decreased after the addition of both 2,4-dcp and cd2+. a larger cd2+ flux was immediately observed after exposure to 0.5 mm cd2+ (−351.25 pmol cm−2 s−1) than to 0.1 mm cd2+ (−107.47 pmol cm−2 s−1). the removal of cd2+ by the ptns increased more after treatment with the 0.5 mm exposure solution (27.6 mg g−1) than with the 0.1 mm exposure solution (3.49 mg g−1).
the enzyme activities were analyzed to review the antioxidative defense system of ptns in a solution containing various concentrations of cd2+. the activities of the coenzyme nicotinamide adenine dinucleotide (nadh) oxidase as well as the enzyme catalase (cat) plateaued at 6.5 u g−1 fw and 9.7 u g−1 fw, respectively, after exposure to 0.25 mm cd2+. the activity of superoxide dismutase (sod) increased gradually in solutions containing 0.1–0.6 mm cd2+, and eventually reached a maximum (68.86 u g−1 fw).
these results illustrate how the antioxidative defense system and the physiological fluxes of ptns respond to the stress caused by toxic pollutants.
中文摘要(谷歌机翻)
负载有tio2纳米粒子(ptns)的固定化phanerochaete chsssporium是用于吸附镉和降解2,4-二氯*(2,4-dcp)的新型高价值生物修复材料。使用非损伤微测技术(nmt)测量h+和o2通量的实时变化。
加入2,4-dcp后,h+流入量增加,而加入cd2+后,h+流入量移出。同时添加2,4-dcp和cd2+后,o2通量下降。暴露于0.5 mm cd2+(-351.25 pmol cm-2 s-1)后立即观察到更大的cd2+通量,而不是暴露于0.1 mm cd2+(-107.47 pmol cm-2 2 s-1)。用0.5 mm暴露溶液(27.6 mg g-1)处理后,ptn去除cd2+的增加比使用0.1 mm暴露溶液(3.49 mg g-1)处理的增加更多。
分析了酶的活性,以审查包含各种浓度的cd2+的溶液中ptn的抗氧化防御系统。暴露于0.25 mm cd2+后,辅酶*腺嘌呤二核苷酸(nadh)氧化酶和过氧化氢酶(cat)的活性分别稳定在6.5 u g-1 fw和9.7 u g-1 fw上。超氧化物歧化酶(sod)的活性在含0.1–0.6 mm cd2+的溶液中逐渐增加,并终达到大值(68.86 u g-1 fw)。
这些结果说明了ptn的抗氧化防御系统和生理通量如何响应有毒污染物引起的压力。
fig. 2. (a) real-time cd2+ flux of ptns exposed to 0.1 mm cd(no3)2 and 10 mg l􀀁1 2,4-dcp.

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