内脂素（VF）检测试剂盒说明书
内脂素(vf)检测试剂盒
适用生物 homo sapiens (human，人)
内脂素(vf)检测试剂盒检测范围 31.25-2000pg/ml 灵敏度 13.5pg/ml
样本类型 serum, plasma and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法 内脂素(vf)检测试剂盒规格 96t
内脂素(vf)检测试剂盒 elisa kit for visfatin (vf)
for in vitro and research use only, not for use in clinical diagnostic procedures!
organism species homo sapiens (human)
product no. sea638hu
sample type serum, plasma and other biological fluids.
format 96-well strip plate
assay length 4.5 hours
detection range 31.25-2000pg/ml the standard curve concentrations used for the elisa’s were 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.25pg/ml
sensitivity the minimum detectable dose of this kit is typically less than 13.5pg/ml.
specificity
this assay has high sensitivity and excellent specificity for detection of visfatin (vf).
no significant cross-reactivity or interference between visfatin (vf) and analogues was observed.
recovery
matrices listed below were spiked with certain level of recombinant visfatin (vf) and the recovery rates were calculated by comparing the measured value to the expected amount of visfatin (vf) in samples.
matrix recovery range (%) average(%)
serum(n=5) 93-104 99
edta plasma(n=5) 99-105 102
heparin plasma(n=5) 79-103 98
precision
intra-assay precision (precision within an assay): 3 samples with low, middle and high level visfatin (vf) were tested 20 times on one plate, respectively.
inter-assay precision (precision between assays): 3 samples with low, middle and high level visfatin (vf) were tested on 3 different plates, 8 replicates in each plate.
cv(%) = sd/meanx100
intra-assay: cv<10%
inter-assay: cv<12%
linearity
the linearity of the kit was assayed by testing samples spiked with appropriate concentration of visfatin (vf) and their serial dilutions. the results were demonstrated by the percentage of calculated concentration to the expected.
sample 1:2 1:4 1:8 1:16
serum(n=5) 80-102% 97-104% 79-101% 93-101%
edta plasma(n=5) 78-102% 98-105% 90-101% 85-103%
heparin plasma(n=5) 91-103% 90-97% 92-101% 80-94%
stability
the stability of kit is determined by the loss rate of activity. the loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
to minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. it is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
reagents and materials provided
reagents quantity reagents quantity
pre-coated, ready to use 96-well strip plate 1 plate sealer for 96 wells 4
standard 2 standard diluent 1×20ml
detection reagent a 1×120μl assay diluent a 1×12ml
detection reagent b 1×120μl assay diluent b 1×12ml
tmb substrate 1×9ml stop solution 1×6ml
wash buffer (30 × concentrate) 1×20ml instruction manual 1
assay procedure summary
1. prepare all reagents, samples and standards;
2. add 100μl standard or sample to each well. incubate 2 hours at 37oc;
3. aspirate and add 100μl prepared detection reagent a. incubate 1 hour at 37oc;
4. aspirate and wash 3 times;
5. add 100μl prepared detection reagent b. incubate 30 minutes at 37oc;
6. aspirate and wash 5 times;
7. add 90μl substrate solution. incubate 15-25 minutes at 37oc;
8. add 50μl stop solution. read at 450nm immediay.
test principle
the test principle applied in this kit is sandwich enzyme immunoassay. the microtiter plate provided in this kit has been pre-coated with an antibody specific to visfatin (vf). standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to visfatin (vf). next, avidin conjugated to horseradish peroxidase (hrp) is added to each microplate well and incubated. after tmb substrate solution is added, only those wells that contain visfatin (vf), biotin-conjugated antibody and enzyme-conjugated avidin will exhibit a change in color. the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. the concentration of visfatin (vf) in the samples is then determined by comparing the o.d. of the samples to the standard curve.
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适用生物 homo sapiens (human，人)
内脂素(vf)检测试剂盒检测范围 31.25-2000pg/ml 灵敏度 13.5pg/ml
样本类型 serum, plasma and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法 内脂素(vf)检测试剂盒规格 96t
内脂素(vf)检测试剂盒 elisa kit for visfatin (vf)
for in vitro and research use only, not for use in clinical diagnostic procedures!
organism species homo sapiens (human)
product no. sea638hu
sample type serum, plasma and other biological fluids.
format 96-well strip plate
assay length 4.5 hours
detection range 31.25-2000pg/ml the standard curve concentrations used for the elisa’s were 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.25pg/ml
sensitivity the minimum detectable dose of this kit is typically less than 13.5pg/ml.
specificity
this assay has high sensitivity and excellent specificity for detection of visfatin (vf).
no significant cross-reactivity or interference between visfatin (vf) and analogues was observed.
recovery
matrices listed below were spiked with certain level of recombinant visfatin (vf) and the recovery rates were calculated by comparing the measured value to the expected amount of visfatin (vf) in samples.
matrix recovery range (%) average(%)
serum(n=5) 93-104 99
edta plasma(n=5) 99-105 102
heparin plasma(n=5) 79-103 98
precision
intra-assay precision (precision within an assay): 3 samples with low, middle and high level visfatin (vf) were tested 20 times on one plate, respectively.
inter-assay precision (precision between assays): 3 samples with low, middle and high level visfatin (vf) were tested on 3 different plates, 8 replicates in each plate.
cv(%) = sd/meanx100
intra-assay: cv<10%
inter-assay: cv<12%
linearity
the linearity of the kit was assayed by testing samples spiked with appropriate concentration of visfatin (vf) and their serial dilutions. the results were demonstrated by the percentage of calculated concentration to the expected.
sample 1:2 1:4 1:8 1:16
serum(n=5) 80-102% 97-104% 79-101% 93-101%
edta plasma(n=5) 78-102% 98-105% 90-101% 85-103%
heparin plasma(n=5) 91-103% 90-97% 92-101% 80-94%
stability
the stability of kit is determined by the loss rate of activity. the loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
to minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. it is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
reagents and materials provided
reagents quantity reagents quantity
pre-coated, ready to use 96-well strip plate 1 plate sealer for 96 wells 4
standard 2 standard diluent 1×20ml
detection reagent a 1×120μl assay diluent a 1×12ml
detection reagent b 1×120μl assay diluent b 1×12ml
tmb substrate 1×9ml stop solution 1×6ml
wash buffer (30 × concentrate) 1×20ml instruction manual 1
assay procedure summary
1. prepare all reagents, samples and standards;
2. add 100μl standard or sample to each well. incubate 2 hours at 37oc;
3. aspirate and add 100μl prepared detection reagent a. incubate 1 hour at 37oc;
4. aspirate and wash 3 times;
5. add 100μl prepared detection reagent b. incubate 30 minutes at 37oc;
6. aspirate and wash 5 times;
7. add 90μl substrate solution. incubate 15-25 minutes at 37oc;
8. add 50μl stop solution. read at 450nm immediay.
test principle
the test principle applied in this kit is sandwich enzyme immunoassay. the microtiter plate provided in this kit has been pre-coated with an antibody specific to visfatin (vf). standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to visfatin (vf). next, avidin conjugated to horseradish peroxidase (hrp) is added to each microplate well and incubated. after tmb substrate solution is added, only those wells that contain visfatin (vf), biotin-conjugated antibody and enzyme-conjugated avidin will exhibit a change in color. the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. the concentration of visfatin (vf) in the samples is then determined by comparing the o.d. of the samples to the standard curve.
ceriliant品牌介绍
SMC5通电磁阀使用方法：
FESTO电磁阀按拦阻外界根源解析
英国普洛帝油液监测家族新品展播一PQ系列铁量仪
回路电阻测试仪的技术指标及性能特点
内脂素（VF）检测试剂盒说明书
显微硬度计的工作原理及应用分享
意大利VESTA气缸怎么选？
同位素气体的性质与应用
高智能土壤养分测速仪应用越来越广泛
纤连蛋白（FN）ELISA现货
漏电开关测试仪 型号:DP-AR5406 资料
潍坊春城环保大中型一体化污水处理设备说明
包装机工作流程的技术要求
活化仪作为配套部件可提高热解吸的工作效率
梧州电影院防火吸音软包厂家
关于码头定点标识航标样式
实验室级别的液相色谱有哪几种类别
荧光酪酰胺缀合物iFluor488Styramide超级信号放大成像试剂盒
关于气缸型号和规格尺寸选型的几个重要的参数